目的 建立猴头菌丝体中15种游离氨基酸的含量测定方法。方法 采用柱前衍生HPLC法测定15种游离氨基酸的含量。使用Ultimate Amino Acid色谱柱(4.6 mm×250 mm, 5 μm), 以流动相A:80%乙腈水溶液, 流动相B:乙酸钠缓冲液(pH 6.5), 梯度洗脱, 流速1.0 mLmin-1, 柱温:40 ℃, 检测波长:254 nm。结果 15种游离氨基酸相关系数为0.999 7~0.999 9, 平均回收率(n=6)在96.83%~98.60%, RSD在0.67%~2.67%。结论 该方法简便、可靠、准确, 可作为猴头菌丝体中氨基酸的含量测定方法。
Abstract
OBJECTIVE To establish a quantitative method of determining the contents of 15 kinds of free amino acids in the Hericium erinaceus mycelium. METHODS The HPLC analysis was performed after derivatization by using phenyl isothiocyanate (PITC) as a derivative reagent. The chromatographic column was Ultimate Amino Acid(4.6 mm×250 mm, 5 μm). The mobile phase A was acetonitrile-water (80∶20), and mobile phase B was sodium acetate buffer (pH 6.5). Gradient elution was performed at the flow rate of 1.0 mLmin-1. The detection wavelength was set at 254 nm, and the column temperature was maintained at 40 ℃. RESULTS The correlation coefficients of 15 kinds of free amino acids ranged from 0.999 7 to 0.999 9. The average recovery rate (n=6) was between 96.83%-98.60%, and the RSD was between 0.67%-2.67%. CONCLUSION This method is simple, accurate and can be used to determine the contents of 15 kinds of free amino acids in the Hericium erinaceus mycelium.
关键词
猴头菌丝体 /
游离氨基酸 /
柱前衍生 /
含量测定
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Key words
Hericium erinaceus mycelium /
free amino acids /
precolumn derivatization /
content determination
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中图分类号：
R284
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